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Approaches to the assessment of matrix turnover in skeletal growth aiming at in vivo monitoring are based on the use of markers of bone formation, including P1NP, osteocalcin and bone alkaline phosphatase, markers of bone resorption, such as CTx. The normal values of these markers are influenced by age, sex, peaking at the end of the growth period, and giving them is based on Calibration of Circulating Markers of Bone Turnover in Skeletal Growth: A Population Projection by Markers on Mathematical Models, in particular the model of Matthiesen et al. based on the "mid to late puberty development" model of Hai et al. At a cross-sectional scan the "normalized Z-score" of the "calibrated serum marker" was found to be significantly deregulated in growing rabbit prostates. The data were used to generate Z-score plots with serially normalization to mean values at a given age or at peak height velocity. The levels of healthy bone turnover in boys and in girls at peak height velocity levels correlate well with the Z-score of the P1NP or CTx (R2 = 0.919, P < 0.001 for both P1NP and CTx).
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